Coding

Part:BBa_K3807017:Design

Designed by: Viktoriia Belousova   Group: iGEM21_KU_Leuven   (2021-10-01)


sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 151
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Taken from a pET29 plasmid supplied by the lab that contains the sfGFP gene. The sfGFP was isolated through PCR, but does not contain a stop codon in its sequence. In our design, the sfGFP is immediately followed by the BioBrick suffix which provides the necessary stop codon.

Source

PCR amplification from plasmids supplied by the lab.

References